05-717

Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2

clone 7F6-G5-A2, Upstate^®, from mouse

• 동의어(들): adenosine A2 receptor, adenosine A2a receptor, adenosine receptor subtype A2a
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 7F6-G5-A2, monoclonal
• 종 반응성: mouse, rat, dog, canine, rabbit, human
• 제조업체/상표: Upstate^®
• 기술: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG2a
• NCBI 수납 번호: NM_000675
• UniProt 수납 번호: P29274
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ADORA2A(135)

설명

일반 설명

Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.

특이성

Cross-reactivity with guinea pig also reported.

This antibody recognizes adenosine receptor A2A, Mr 43 kDa.

면역원

6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. Clone 7F6-G5-A2.

애플리케이션

Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2 is an antibody against Adenosine Receptor A2a clone 7F6-G5-A2 for use in FC, IH & WB.

Immunohistochemistry: 1 μg/mL of a previous lot detected Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice.

FACS Analysis: A previous lot of this antibody has been reported by an independent laboratory to detect Adenosine Receptor A2A in transfected HEK293 cells (Koshiba, M., 1999).

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Neurodegenerative Diseases

품질

Routinely evaluated by immunohistochemistry.

Western Blot Analysis:
A 1:1000–1:5000 dilution of this lot of antibody detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras (Rosin, D.L., 1998).

표적 설명

~43 kDa

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt

분석 메모

Control
Brain tissue

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable