475989

MTT

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

• 동의어(들): MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
• 실험식(Hill 표기법): C₁₈H₁₆BrN₅S
• CAS Number: 298-93-1
• Molecular Weight: 414.32
• MDL number: MFCD00011964
• UNSPSC 코드: 12171500
• NACRES: NA.25

속성

• Quality Level: 100
• 설명: RTECS - XF8060000
• 분석: ≥98% (silver nitrate titration)
• 양식: solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 불순물: ≤0.5% Sulfated ash
• 색상: yellow
• solubility: PBS: 2 mg/mL
• 양이온 미량물: heavy metals: ≤0.001%
• 배송 상태: ambient
• 저장 온도: 15-25°C
• SMILES string: [s]1c(nc(c1C)C)[n]2[n+](nc(n2)c4ccccc4)c3ccccc3.[Br-]
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChI key: AZKSAVLVSZKNRD-UHFFFAOYSA-M

설명

일반 설명

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.

생화학적/생리학적 작용

Cell permeable: yes

Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.

Product does not compete with ATP.

Reversible: no

경고

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

재구성

Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.

기타 정보

Liu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H315,H319,H335,H341
• 예방조치 성명서: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable