AB16926

Anti-DFF40 Antibody

Chemicon^®, from rabbit

• 동의어(들): DNA Fragmentation Factor, 40 kDa Subunit, Caspase-activated DNase, CAD, CPAN
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, mouse, rat
• 제조업체/상표: Chemicon^®
• 기술: western blot: suitable
• NCBI 수납 번호: NM_001004285.1; NM_001004286.1; NM_004402.2
• UniProt 수납 번호: O76075
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... DFFB(1677)

설명

특이성

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (Enari et al. 1998; Sakahira et al. 1998). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN and DFF40 and human CAD, respectively (Halenbeck et al. 1998; Liu et al. 1998; Mukae et al. 1998). DFF45/ICAD is the inhibitory protein of DFF40/CAD (Enari et al. 1998; Sakahira et al. 1998; Liu et al. 1997) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually cuases the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

면역원

A peptide corresponding to amino acids 147-164 of mouse CAD (Enari et al. 1998). The sequence differs from human DFF40 by two amino acids (Halenbeck et al. 1998; Liu et al. 1998).

애플리케이션

Detect DFF40 using this Anti-DFF40 Antibody validated for use in WB.

Western blot: 1:500-1:1,000

K562 or Jurkat whole cell lysate can be used as a positive control and a 40 kDa band should be detected.

Optimal working dilutions must be determined by end user.

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable