ABE457

Anti-c-Fos Antibody

rabbit polyclonal

• 동의어(들): Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• Product Name: Anti-c-Fos Antibody, from rabbit, purified by affinity chromatography
• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, rat
• 종 반응성(상동성에 의해 예측): sheep (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology)
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NP_005243
• UniProt 수납 번호: P01100
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FOS(2353)

설명

일반 설명

c-Fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-Fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

특이성

This antibody recognizes the N-terminus of c-Fos.

면역원

KLH-conjugated linear peptide corresponding to the N-terminus of human c-Fos.

애플리케이션

Anti-c-Fos Antibody is a Rabbit Polyclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB & IHC.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-Fos in rat pons and rat cerebellum tissues.

Immunofluorescence Analysis: A representative lot detected c-Fos in rat pons and rat cerebellum tissues.

품질

Evaluated by Western Blot in PMA(TPA) treated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos in 10 µg of PMA(TPA) treated HeLa cell lysate.

표적 설명

~60/56 kDa observed. This protein has multiple isoforms produced by alternative splicing ranging between 53 and 68 kDa (Jurado, J., et al. (2007). BMC Molecular Biology. 8:83-96.)

분석 메모

Control
PMA(TPA) treated HeLa cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable