추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
monkey, mouse
종 반응성(상동성에 의해 예측)
rat (based on 100% sequence homology), human (based on 100% sequence homology)
기술
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... TUBA1A(7846)
일반 설명
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
특이성
This antibody detects the tyrosinated form of alpha Tubulin.
면역원
KLH-conjugated linear peptide corresponding to human tyrosinated alpha Tubulin.
애플리케이션
Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, tyrosinated validated for use in western blotting, ELISA & ICC.
Western Blotting Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin and not nontyrosinated alpha Tubulin using purified porcine tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
ELISA Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin, in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
ELISA Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin, in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
품질
Evaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, tyrosinated in 10 µg of untreated NIH/3T3 cell lysate and demonstrated a loss of signal in PCA treated NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, tyrosinated in 10 µg of untreated NIH/3T3 cell lysate and demonstrated a loss of signal in PCA treated NIH/3T3 cell lysate.
표적 설명
~51 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
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