LIF2010
Leukemia Inhibitory Factor from mouse
10 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
동의어(들):
LIF, Differentiation-stimulating Factor, D Factor, Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF
로그인조직 및 계약 가격 보기
모든 사진(1)
About This Item
UNSPSC 코드:
12352202
eCl@ss:
32160405
NACRES:
NA.75
추천 제품
생물학적 소스
mouse
Quality Level
분석
>95% (HPLC and SDS-PAGE)
양식
liquid
특이 활성도
≥1 x 10(E8) U/mg
제조업체/상표
Chemicon®
농도
10 μg/mL
기술
cell culture | stem cell: suitable
불순물
<0.1 ng/mg Endotoxin (of LIF)
입력
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
애플리케이션
RECOMMENDED QC PROTOCOL
M1 Bioassay
1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
3. Add 100 μL of 5% FCS in isotonic saline to two control slides.
4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
Visit www.esgro-lif.com for additional information
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.
M1 Bioassay
1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
3. Add 100 μL of 5% FCS in isotonic saline to two control slides.
4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
Visit www.esgro-lif.com for additional information
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.
단위 정의
Specific Activity: where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
분석 메모
Specific Activity: The activity of mouse LIF is determined by the ability to induce differentiation of murine M1 myeloid leukemic cells.The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL
Tested negative in both aseptic and microplasmic tests.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.