MAB16985

Anti-MCAM Antibody, clone P1H12

clone P1H12, Chemicon^®, from mouse

• 동의어(들): CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: P1H12, monoclonal
• 종 반응성: mouse, canine, human
• 반응하면 안 됨: rat
• 포장: antibody small pack of 25 μg
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1
• 적합성: not suitable for immunohistochemistry (Paraffin)
• NCBI 수납 번호: NM_006500.2
• UniProt 수납 번호: P43121
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MCAM(4162)

설명

일반 설명

MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).

특이성

Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.

면역원

Immunization of mice with HUVEC.

애플리케이션

Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.

Immunocytochemistry:
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.

Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.

Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.

ELISA:
1-10 μg/mL of a previous lot worked in ELISA.

FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.

Optimal working dilutions must be determined by end user.

품질

Routinely evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.

표적 설명

110 kDa

물리적 형태

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.

분석 메모

Control
HUVEC cells.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable