MABT201

Anti-Integrin alpha 5 (ITGA5/CD49E) Antibody

mouse monoclonal, SNAKA51

• 동의어(들): Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• Product Name: Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51, clone SNAKA51, from mouse, purified by using protein G
• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: SNAKA51, monoclonal
• 정제법: using protein G
• 종 반응성: human
• 기술: activity assay: suitable; immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2α
• NCBI 수납 번호: NP_001138472
• UniProt 수납 번호: P08648
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ITGAV(3685)

설명

일반 설명

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The α5/β1 integrin, also termed VLA5 or CD49e, is identical to the fibronectin receptor and mediates the adhesion of cells to fibronectin.

면역원

Whole cells corresponding to human Integrin alpha5.

애플리케이션

Activity Assay: A representative lot of this antibody converts alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.).
Immunocytochemistry Analysis: A representative lot of this antibody detected Integin alpha 5 in Primary human foreskin fibroblasts (hFF) (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.; Valdembri, D., et al. (2009). PLoS Biol. 7(1):e25.).

Detect Integrin alpha5 (Preservative Free) using this Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51 validated for use in Western Blotting, Activity Assay, ICC.

Research Category
Cell Structure

품질

Evaluated by Flow Cytometry in HeLa cell treated with fibronectin.
Flow Cytometry Analysis: 1 µg of this antibody detected Integrin alpha5 in 0.5X10^6 HeLa cells.

표적 설명

116 kDa calculated

물리적 형태

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.

분석 메모

Control
HeLa cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable