OP33

Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

liquid, clone PAb1620, Calbiochem^®

• 동의어(들): Anti-p53 Antibody, Mouse Anti-p53, p53 Detection Antibody
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: PAb1620, monoclonal
• 양식: liquid
• 포함: ≤0.1% sodium azide as preservative
• 종 반응성: human, mouse, rat, primate, bovine
• 제조업체/상표: Calbiochem^®
• 저장 조건: do not freeze
• 동형: IgG2a
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TP53(7157)

설명

일반 설명

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~53 kDa wild-type p53 protein.

Recognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.

This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).

면역원

vLM mouse tumor cells

애플리케이션

Frozen Sections (see applications references)

Immunoblotting (not recommended)

Immunofluorescence (1-20 μg/ml)

Immunoprecipitation (1 μg per sample)

Paraffin Sections (5 μg/ml, heat pre-treatment required)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

분석 메모

Positive Control
Hs27 cells or breast carcinoma tissue

기타 정보

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Ball, R.K., et al. 1984. EMBO J.3, 1485.

Wild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with ³⁵S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable