10104159001

DNase I

grade II, from bovine pancreas

• 동의어(들): Deoxyribonuclease, Deoxyribonucleate 5′-oligonucleotido-hydrolase
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bovine pancreas
• Quality Level: 100
• 양식: lyophilized
• 포장: pkg of 100 mg
• 제조업체/상표: Roche
• 기술: DNA extraction: suitable
• 최적 pH: ~7.0
• 저장 온도: 2-8°C

설명

일반 설명

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.

애플리케이션

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-

• The isolation of cells from lung, skin and tumor samples
• Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †

단위 정의

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

제조 메모

Activator: Ca²⁺ (0.12 mM) in combination with Mg²⁺ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash