10108090001

Peroxidase (POD)

grade I, from horseradish

• 동의어(들): POD, peroxidase
• EC 번호: 1.11.1.7 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: horseradish
• Quality Level: 100
• 양식: lyophilized (salt-free)
• 특이 활성도: ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.); 250 U/mg
• 포장: pkg of 25,000 U
• 제조업체/상표: Roche
• 최적 pH: 6.0-6.5
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Donor:hydrogen-peroxide oxidoreductase

Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.

애플리케이션

Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.

Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H₂O₂ produced from disaggregated slug cells.

생화학적/생리학적 작용

Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.

품질

Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase
Purity number: approximately 3.0 (A₄₀₃/A₂₇₅)

제조 메모

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).

재구성

Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable