10197734001

Glutamate Dehydrogenase (GlDH)

from beef liver

• 동의어(들): DNA marker
• EC 번호: 1.4.1.3 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bovine liver
• Quality Level: 100
• 양식: lyophilized
• 특이 활성도: 10 U/mg; ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
• 포장: pkg of 3,000 U
• 제조업체/상표: Roche
• 농도: ≥10-20 % (w/w)
• 기술: activity assay: suitable
• 색상: white
• 최적 pH: 8
• solubility: water: 20 mg/mL, colorless
• 적합성: suitable for UV spectrophotometry and general use
• UniProt 수납 번호: P00366
• 응용 분야: life science and biopharma
• 외래 활성: ADH <0.00500%; LDH <0.00500%; MDH <0.00500%
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... GLUD1(281785)

설명

일반 설명

Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.

GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)⁺ oxidoreductase is involved in deamination.

애플리케이션

Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.

품질

Contaminants: <0.005% ADH, LDH, and MDH, each

서열

The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash