10745782001

DNA, lambda

from bacteriophage lambda cl857 Sam 7

• 동의어(들): lambda dna
• UNSPSC 코드: 41106310

속성

• Quality Level: 100
• 양식: solution
• 분자량: 32,300 kDa
• 포장: pkg of 1 mL
• 제조업체/상표: Roche
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 250 μg/mL
• 불순물: RNA, none detected; nuclease, none detected
• 색상: colorless
• solubility: water: miscible
• 배송 상태: dry ice
• 저장 온도: −20°C (−15°C to −25°C)

설명

일반 설명

The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.

애플리케이션

DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.

품질

The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.

서열

Chain Length 48,502 bp

물리적 형태

Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use

저장 및 안정성

Store at -15 to -25°C until use; once thawed, store at 2-8 °C.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): No data available
• Flash Point (°C): No data available