11388908910

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

• 동의어(들): Biotin-16-UTP, biotin
• UNSPSC 코드: 41116100

속성

• 분석: 97.7% (HPLC)
• Quality Level: 100
• 양식: solution
• 분자량: M_r 987.5 (biotin-16-UTP-Li₄)
• 포장: pkg of 25 μL (250 nmol; 10mM)
• 제조업체/상표: Roche
• 색상: colorless
• solubility: water: miscible
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

애플리케이션

Biotin-labeled RNA can be used as a hybridization probe for:

• Northern blots
• Southern blots
• Plaque or colony lifts
• RNase protection experiments
• In situ hybridization
• Microarray hybridization

The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

품질

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

물리적 형태

Biotin-16-UTP, tetralithium salt, 10mM solution

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash