11685619910

Fluorescein RNA Labeling Mix

solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization

• 동의어(들): RNA labeling
• UNSPSC 코드: 41105500

속성

• 양식: solution
• Quality Level: 100
• 사용: sufficient for 20 reactions
• 포장: pkg of 40 μL
• 제조업체/상표: Roche
• 기술: Northern blotting: suitable; Southern blotting: suitable; hybridization: suitable
• 저장 온도: −20°C

설명

일반 설명

Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).

특이성

Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).

애플리케이션

Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:

• in situ hybridization to tissues
• Southern blots
• northern blots
• plaque or colony lifts
• RNase protection experiments

품질

The mix is function tested in combination with the DIG RNA Labeling Kit.

원리

Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.

제조 메모

10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): No data available
• Flash Point (°C): No data available