CK-RO

Creatine Kinase (CK)

from rabbit muscle

• 동의어(들): creatine phosphokinase
• EC 번호: 2.7.3.2 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: rabbit muscle
• Quality Level: 100
• 양식: lyophilized
• 포장: pkg of 100 mg (10127566001); pkg of 500 mg (10736988001)
• 제조업체/상표: Roche
• 색상: white to light yellow
• 최적 pH: 6.0-7.0(reverse reaction); 9.0(forward reaction)
• solubility: water: soluble
• 적합성: suitable for immunoprecipitation
• NCBI 수납 번호: NP_001075708
• UniProt 수납 번호: P00563
• 응용 분야: life science and biopharma; sample preparation
• 외래 활성: ATPase <0.001%; HK <0.001%; myokinase <0.001%
• 저장 온도: 2-8°C
• 유전자 정보: rabbit ... CKM(100009056)

설명

일반 설명

at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.

애플리케이션

Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.

생화학적/생리학적 작용

Creatine kinase (CK) is a guanidino-kinase that catalyzes the reversible phosphorylation of creatine to phosphocreatine at the expense of ATP. It plays crucial roles in rapid regeneration of ATP in cells with high demand for energy. Increased level of CK in human blood indicates diseases of the nervous system and the heart muscle, malignant hypothermia, and certain tumors. Metals such as Mg, Mn and Ca act as cofactors for CK. It also forms a substrate for protein kinase C, an important signaling molecule involved in cell growth. A product of enzymatic reaction of CK, phosphocreatine, is found to prevent HIV (human immunodeficiency virus) replication in macrophages.

품질

Contaminants: <0.001% ATPase, HK, and myokinase, each

규격

Specific activity: Approximately 350U/mg lyophilizate at +25°C (800U/mg lyophilizate at +37°C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as activator.

단위 정의

One unit CK activity (+25 °C) = 2.3 U (+37 °C), creatin-P and ADP as substrates, NAcCys as reactivator.

물리적 형태

Lyophilizate, stabilized

제조 메모

Storage conditions (working solution): Working solutions and storage conditions in routine applications:
Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C.
Stable for approximately four weeks at -15 to -25 °C.
Avoid repeated freezing and thawing.

Dissolve in 0.5% NaHCO3 solution.
Do not freeze. Store protected from light.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Or
Dissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.
Stable for 2 - 3 days, when stored at 2 to 8 °C.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash