DNALIG-RO

T4 DNA Ligase

solution, from Escherichia coli(NM 989), suitable for activity assay

• 동의어(들): dna ligase, t4
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: Escherichia coli (NM 989)
• Quality Level: 100
• 재조합: expressed in E. coli
• 설명: recombinant form of the enzyme from T4 phage
• 양식: solution
• 특이 활성도: ~3000 units/mg protein
• 포장: pkg of 100 U (10481220001 [1 U/μl]); pkg of 500 U (10716359001 [1 U/μl]); pkg of 500 U (10799009001 [5 U/μl])
• 제조업체/상표: Roche
• 파라미터: 4-22 °C optimum reaction temp.
• 기술: activity assay: suitable
• 최적 pH: 7.2-7.8
• 저장 온도: −20°C

설명

일반 설명

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.

Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.

특이성

Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).

애플리케이션

Ligation of sticky- and blunt-ended DNA fragments.

품질

Quality

Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.

단위 정의

One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol ³²P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 μg/ml, pH 7.6 (+25 °C).

Volume Activity: 1 x 10³ U/ml; 5 x 10³ U/ml

제조 메모

Activator: Mg²⁺ (10mM), DTT
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash