FIBRP-RO

Fibronectin (pure)

from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)

• 동의어(들): Fibronectin
• MDL number: MFCD00131062
• UNSPSC 코드: 12352202

속성

• 생물학적 소스: human plasma
• Quality Level: 100
• 무균: non-sterile; 0.2 μm filtered
• 분석: >95% (SDS-PAGE)
• 양식: lyophilized (clear, colorless solution after reconstitution)
• 분자량: 440 kDa
• 포장: pkg of 1 mg (11051407001); pkg of 5 mg (11080938001)
• 제조업체/상표: Roche
• 기술: cell culture | mammalian: suitable
• UniProt 수납 번호: P02751
• 저장 온도: 2-8°C
• 유전자 정보: human ... FN1(2335)

설명

일반 설명

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

특이성

Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).

애플리케이션

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.

서열

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

물리적 형태

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

제조 메모

Working concentration: 5μg/cm² for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

재구성

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

기타 정보

For life science research only. Not for use in diagnostic procedures.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash