B4380

Bromobimane

≥97% purity, powder

• 동의어(들): Monobromobimane
• 실험식(Hill 표기법): C₁₀H₁₁BrN₂O₂
• CAS Number: 71418-44-5
• Molecular Weight: 271.11
• Beilstein: 4430959
• MDL number: MFCD00036951
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24891769
• NACRES: NA.47

속성

• Product Name: Bromobimane, ≥97% (HPLC)
• Quality Level: 200
• 분석: ≥97% (HPLC)
• 양식: powder
• 색상: yellow
• mp: 161 °C
• solubility: acetonitrile: 20 mg/mL
• ε (흡광계수): 4.6-5.1 at 396-398 nm in H₂O
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: −20°C
• SMILES string: CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
• InChI: 1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
• InChI key: AHEWZZJEDQVLOP-UHFFFAOYSA-N

설명

일반 설명

Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

애플리케이션

Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria.
Bromobimane has been used for the quantitative measurement of free hydrogen sulfide in vivo and in vitro. It has been used for the labeling of proteins containing thiol groups.

생화학적/생리학적 작용

Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH and upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)