P1379

TWEEN^® 20

viscous liquid

• 동의어(들): Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
• CAS Number: 9005-64-5
• MDL number: MFCD00165986
• UNSPSC 코드: 12161900
• NACRES: NA.21

속성

• 설명: non-ionic
• Quality Level: 300
• 분석: >40% (GC)
• 양식: viscous liquid
• 분자량: ~1228
• 구성: lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
• 농도: ≥40.0% (GC)
• 기술: DNA extraction: suitable; RNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• 불순물: ≤3.0% water
• refractive index: n20/D 1.468 (lit.)
• CMC: 0.06 mM (20-25°C)
• 산성 번호: ≤2.2 mg/g
• 수산기 값: 96‑108 mg/g
• 전이 온도: cloud point 76 °C
• density: 1.095 g/mL at 25 °C (lit.)
• HLB: 16.7
• 적합성: complies for IR spectroscopy
• SMILES string: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
• InChI key: HMFKFHLTUCJZJO-UHFFFAOYSA-N

설명

일반 설명

Tween^® 20 is a nonionic surfactant and detergent widely applied in biochemical and life science research. Its predominant use involves the solubilization of membrane proteins during the isolation of membrane-protein complexes. Moreover, it serves as a component in various washing, blocking, dilution, and stabilization buffers. Additionally, it functions as a reagent for immunohistochemistry, encompassing techniques like Western blotting and ELISAs. In these applications, Tween 20 plays crucial roles such as preventing non-specific antibody binding, minimizing background staining, and ensuring the even distribution of reagents.

Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

애플리케이션

Non-ionic detergent

TWEEN 20 has been used as a component of:

• washing buffer and blocking buffer in Western blotting
• blocking buffer in immunohistochemistry
• washing buffer in ELISA (enzyme linked immunosorbent assay)
• reaction mixture in PCR (polymerase chain reaction)

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

특징 및 장점

• Highly versatile surfactant for your life science and biochemical research
• Excellent solubilising, dispersing and emulsifying agent
• Suitable for DNA & RNA extraction, mammalian cell culture, and protein purification

포장

P1379-1KT:
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.

제조 메모

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

기타 정보

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법적 정보

TWEEN is a registered trademark of Croda International PLC

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 527.0 °F - Pensky-Martens closed cup
• Flash Point (°C): 275 °C - Pensky-Martens closed cup
• 개인 보호 장비: Eyeshields, Gloves