추천 제품
Quality Level
양식
solution
재고 정보
not available in China
농도
1.5 mg/mL in H2O
색상
colorless to light brown
적합성
suitable for (Fluorescent detection of gram-negative bacteria)
suitable for (Microbiome)
suitable for microbiology
동작 모드
cell membrane
배송 상태
ambient
저장 온도
−20°C
일반 설명
Dansyl Polymyxin B (DSL-PMB) is a fluorescent derivative of Polymyxin B. Polymyxin B is a cationic lipopeptide antibiotic that binds specifically to Gram-negative bacteria. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.
Dansyl labeled Polymyxin B can be utilized for fluorescent microbial imaging in the fields of Cell Biology and Biochemical research.
애플리케이션
DSL-PMB may have been used:
- as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics
- in research and to develop new active derivatives of Polymyxin against multi-drug resistance Gram-negative infections
- to measure LPS binding affinity by fluorometric displacement assay
- Antimicrobial resistance research.
- Bacterial visualization and imaging.
- Parent antibiotic mode of action research and new antibiotic discovery.
- Toxicity studies.
- Research of bacterial infections and tracking its uptake in vivo.
생화학적/생리학적 작용
DSL-PMB′s mode of action is in accordance with Polymyxin B activity. However, MIC assays comparing DSL-PMB, and parent Polymyxin B showed a two to three-fold decrease in bacterial activity. Polymyxin B interacts with anionic lipopolysaccharide (LPS) molecules which are located in Gram-negative bacterial outer membrane. This interaction interferes with the outer membrane′s normal function, causing leakage and enhanced uptake of the antibiotic. The resemblance in properties between Polymyxin B and DSL-PMB allows to fluorescently label Gram-negative bacteria and examine intracellular localization and penetration of Polymyxins in Gram-negative bacteria.
특징 및 장점
- High-quality antibiotic suitable for multiple research applications
- Ideal for Cell Biology, Metabolomics, and Biochemical research.
분석 메모
- Dansyl Labeled Polymyxin B Ready Made Solution is light sensitive.
- It is recommended to avoid freeze-thaw cycles of PMB-DSL Ready Made Solution.
- Dansyl Labeled Polymyxin B Ready Made Solution (1.5 mg/mL) can be diluted 1:50 in PBSX1 (Sigma#D8537) to achieve 30 μg/mL final concentration for staining. The above concentration of DSL-PMB was used for E. coli staining see image.
- Fluorescence Microscopy application: Dansyl Labeled Polymyxin B Ready Made Solution excitation (Ex) wavelength is 330-340nm resulting in emission (Em) range of 540-600nm (λmax=570nm).
기타 정보
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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