19899

CHAPS 100 mM solution

• 동의어(들): CHAPS solution
• 실험식(Hill 표기법): C₃₂H₅₈N₂O₇S
• CAS Number: 75621-03-3
• Molecular Weight: 614.88
• MDL number: MFCD00012116
• UNSPSC 코드: 12161902
• PubChem Substance ID: 57647807
• NACRES: NA.25

속성

• 설명: zwitterionic
• Quality Level: 100
• 양식: liquid
• 분자량: 614.88 g/mol
• 농도: 100 mM in H₂O
• 기술: protein quantification: suitable
• CMC: 8 mM
• 전이 온도: cloud point >100 °C
• density: 1.01 g/mL at 20 °C
• 저장 온도: 2-8°C
• SMILES string: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• InChI key: UMCMPZBLKLEWAF-RFCNGIAKSA-N

설명

일반 설명

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.

애플리케이션

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.

생화학적/생리학적 작용

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves