23479

Nitrite/Nitrate Assay Kit, colorimetric

used for detection of nitric oxide metabolites

• 동의어(들): Nitrate/Nitrite Assay Kit, colorimetric
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• Quality Level: 100
• 사용: sufficient for 100 assays
• 적합성: passes test for application
• 저장 온도: 2-8°C

설명

애플리케이션

Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.

The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO₂, and naphthylethylenediamine. The NO₂/NO₃ Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO₂, NO₃ solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO₂ detection range is from 10 to 100 μM.

키트 구성품 전용

• NaNO₂ Standard Solution (100 mM) 1 bottle
• NaNO₃ Standard Solution (100 mM) 1 bottle
• Buffer solution (20 mM, pH 7.6) 1 bottle
• Nitrate reductase (lyophilized) 1 bottle
• Enzyme Co-factor (lyophilized) 1 bottle
• Griess Reagent A 1 bottle
• Griess Reagent B 1 bottle

안전성 정보

• 픽토그램: GHS05,GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H290,H315,H317,H319,H335
• 예방조치 성명서: P234 - P261 - P264 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable