40709

Atto 590-Streptavidin

BioReagent, suitable for fluorescence

• 동의어(들): Streptavidin-Atto 590
• MDL number: MFCD04040679
• UNSPSC 코드: 12352108
• NACRES: NA.32

속성

• 제품 라인: BioReagent
• Quality Level: 100
• 양식: powder
• 제조업체/상표: ATTO-TEC GmbH
• 형광: λ_ex 590 nm; λ_em 619 nm in 0.1 M phosphate pH 7.0
• 적합성: suitable for fluorescence
• 저장 온도: −20°C

설명

애플리케이션

Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Atto 590 has been coupled to streptavidin to enable all kinds of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays. Streptavidin conjugation of Atto 590 does not significantly change the labels spectral data regarding excitation and emission maxima. Given the optimized Dye/Protein-ratio molar absorbance and quantum yields remain high within application. Conjugates of our Atto dyes generally do not show strong fluorescence in unbound state, but fluorescence is boosted again as soon as they are bound to biotin. This may help overcome problems by unspecific binding which may sometimes appear with streptavidin based assays in special cases.

분석 메모

free of unconjugated dye

법적 정보

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)