77160

Pepsin from porcine gastric mucosa

powder, slightly beige, ≥500 U/mg

• 동의어(들): Pepsin A, Pepsin from hog stomach
• CAS Number: 9001-75-6
• EC 번호: 3.4.23.1 (BRENDA, IUBMB)
• EC Number: 232-629-3
• MDL number: MFCD00081840
• UNSPSC 코드: 12352204
• eCl@ss: 42010127
• NACRES: NA.54

속성

• 양식: powder
• 특이 활성도: ≥500 U/mg
• 분자량: 35 kDa
• 색상: slightly beige
• UniProt 수납 번호: P00791
• 저장 온도: 2-8°C
• 유전자 정보: pig ... LOC396892(396892)

설명

애플리케이션

Pepsin cleavage can be used to produce F(ab′)₂ fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

생화학적/생리학적 작용

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

단위 정의

One unit corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37 °C (Hemoglobin, Catalog No. 51290, as substrate); 15,000 absorbance units as described above are equivalent to ∼1 Bergmeyer unit. One Bergmeyer unit is the amount of enzyme which hydrolyzes 1 μmole Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37 °C.

분석 메모

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

기타 정보

Peptide synthesis,citationcitation Review.citation

Peptide synthesis, Review.

면책조항

Sales restrictions may apply.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves