79760

Phthaldialdehyde

≥99.0% (HPLC)

• 동의어(들): Benzene-1,2-dicarboxaldehyde, OPA, o-Phthalaldehyde, o-Phthalic dicarboxaldehyde, o-Phthalaldehyde, o-Phthalic dicarboxaldehyde
• 실험식(Hill 표기법): C₈H₆O₂
• CAS Number: 643-79-8
• Molecular Weight: 134.13
• Beilstein: 878317
• EC Number: 211-402-2
• MDL number: MFCD00003335
• UNSPSC 코드: 12352201
• PubChem Substance ID: 57652816
• NACRES: NA.32

속성

• Quality Level: 100
• 분석: ≥99.0% (HPLC)
• 양식: solid
• 불순물: ≤0.5% phthalic acid
• mp: 54-56 °C
• 형광: λ_ex 334 nm; λ_em 455 nm (Thiol Adduct); λ_ex 340 nm; λ_em 450 nm in reaction buffer (with glycine)
• 적합성: suitable for fluorescence
• 저장 온도: 2-8°C
• SMILES string: O=Cc1ccccc1C=O
• InChI: 1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
• InChI key: ZWLUXSQADUDCSB-UHFFFAOYSA-N

설명

일반 설명

Phthaldialdehyde, also known as o-Phthalaldehyde or OPA, is a pre-column (HPLC) derivatization reagent. OPA reacts with primary amino acids (AA) except cystine to form highly fluorescent adducts. The fluorescence of the derivatives is monitored at excitation and emission wavelengths of 345nm and 455nm, respectively. The HPLC technique efficiently separates the OPA-AA derivatives.

애플리케이션

Phthaldialdehyde or OPA determines amino acids in proteins from animal tissue and food samples. Quantification of amino acids adducts of tyramine and phenylethylamine produced by lactic acid bacteria used OPA as the pre-column derivative reagent for HPLC. Intracellular forms of glutathione (GSH/GSSG) are investigated using o-Phthalaldehyde as the pre-column fluorescent agent.

생화학적/생리학적 작용

Phthaldialdehyde can be used for the pre-column derivatization of amino acids for high-performance liquid chromatography (HPLC) separation. It may also be used for flow cytometric measurements of protein thiol groups.

안전성 정보

• 픽토그램: GHS06,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H301,H314,H317,H335,H410
• 예방조치 성명서: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): 269.6 °F - closed cup
• Flash Point (°C): 132 °C - closed cup
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter