81349

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt

free from low molecular weight nucleotides, lyophilized

• 동의어(들): Poly(dI-dC) sodium salt
• CAS Number: 118578-37-3
• MDL number: MFCD00147947
• UNSPSC 코드: 12352106
• NACRES: NA.52

속성

• Grade: Molecular Biology
• Quality Level: 100
• 양식: powder
• 품질: lyophilized
• 분자량: M_r ~2.5 × 10⁶ (broad molecular weight distribution); M_r ~2500000
• 포장: ampule of ≥10 units A₂₆₀
• 저장 온도: −20°C

설명

일반 설명

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

애플리케이션

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.

Suitable for

• non-specific blocking agent in EMSA
• evaluation of DNA methyltransferases
• DNA-small molecule or drug interaction studies

생화학적/생리학적 작용

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)