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A2231

Sigma-Aldrich

Anti-Axin1 (C-terminal region) antibody produced in rabbit

~2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-Axin, Anti-Axis Inhibitor 1, Anti-Axis inhibition protein 1

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41
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기술 서비스
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도움 문의

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

buffered aqueous solution

분자량

antigen ~130 kDa

종 반응성

mouse, rat, human

농도

~2.5 mg/mL

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
Y4770WH0008312M1SAB3500619
biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

technique(s)

immunohistochemistry: suitable, western blot: 0.5-1 μg/mL using HEK-293 cells expressing mouse axin1

technique(s)

western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and c-Abl

technique(s)

immunofluorescence: suitable, indirect ELISA: suitable, proximity ligation assay: suitable, western blot: 1-5 μg/mL

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

일반 설명

Axin1 contains two conserved domains, an N-terminal regulator of G-protein signaling (RGS) domain, and a C-terminal DIX domain. The C-terminal region of axin1 is important for homodimerization, whereas the central region binds β-catenin and GSK-3b.

면역원

synthetic peptide corresponding to amino acids 815-833 located near the C-terminal region of human axin1, conjugated to KLH. This sequence is identical in rat and mouse axin1 and highly conserved (83% identity) in human, rat, and mouse axin2.

애플리케이션

Immunohistochemistry was performed using rabbit anti-axin antibody to visualize Axin in the mouse developing cortex. Mouse brains had been previously harvested and fixed in 4% paraformaldhyde and cryoprotected in 20% sucrose. Axin was seen in the soma and axons of neurons.
Rabbit anti-axin1 (C-terminal region) antibody can be used at a concentration of 0.5-1μg/ml in western blot assays using HEK-293 cells expressing mouse axin1.

생화학적/생리학적 작용

Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC).
Axin1 (axis inhibitor-1, axin) enhances the phosphorylation of β-catenin by Glycogen synthase kinase 3 β (GSK-3b). Axin1 is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3b thus, facilitating β-catenin phosphorylation by GSK-3b. Axin1 itself is phosphorylated and its activity regulated by GSK-3b. Axin1 phosphorylation is reduced by Wnt signaling, leading to decreased affinity of interaction of axin1 and β-catenin. This results in the release of β-catenin from the degradation complex and increased stability of β-catenin in the cell. Axin1 is destabilized by Wnt signaling and its levels in the cell are reduced. Axin isoform axin2 (conductin, axil) is 44% identical to Axin1 and appears to play a similar role to Axin1 in the Wnt signaling pathway.

물리적 형태

Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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