A2714

Aldolase from rabbit muscle

lyophilized powder, ≥8.0 units/mg protein

• 동의어(들): D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase
• CAS Number: 9024-52-6
• EC 번호: 4.1.2.13 (BRENDA, IUBMB)
• EC Number: 232-781-0
• MDL number: MFCD00130453
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: rabbit muscle
• Quality Level: 300
• 양식: lyophilized powder
• 특이 활성도: ≥8.0 units/mg protein
• 구성: Protein, ≥80% biuret
• 외래 활성: glyceraldehyde-3-phosphate dehydrogenase ≤0.03%; lactic dehydrogenase ≤0.03%; phosphoglucose isomerase ≤0.6%; pyruvate kinase ≤0.1%; triosephosphate isomerase ≤0.05%
• 저장 온도: −20°C

설명

애플리케이션

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 . Product A2714 is essentially sulfate-free and contains citrate buffer salts.

생화학적/생리학적 작용

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold .

단위 정의

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

물리적 형태

Essentially sulfate-free containing citrate buffer salts

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)