A4362

Activin A human

Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE), suitable for cell culture

• 동의어(들): Erythroid differentiation protein, Inhibin beta A chain; Activin beta-A chain
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• Product Name: Activin A active human, Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in Nicotiana
• 분석: >97% (SDS-PAGE)
• 양식: lyophilized
• 효능: ≤5 ng/mL ED₅₀
• 분자량: 27.4 kDa (disulfide-linked homodimers of two βA chains, each containing 116 amino residues)
• 포장: pkg of 5 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 불순물: Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
• UniProt 수납 번호: P08476
• 저장 온도: −20°C
• 유전자 정보: human ... INHBA(3624)

설명

Amino Acid Sequence

HisHisHisHisHisHisGlyLeuGluCysAspGlyLysValAsnIleCysCysLysLysGlnPhePheValSerPheLysAspIleGlyTrpAsnAspTrpIleIleAlaProSerGlyTyrHisAlaAsnTyrCysGluGlyGluCysProSerHisIleAlaGlyThrSerGlySerSerLeuSerPheHisSerThrValIleAsnHisTyrArgMetArgGlyHisSerProPheAlaAsnLeuLysSerCysCysValProThrLysLeuArgProMetSerMetLeuTyrTyrAspAspGlyGlnAsnIleIleLysLysAspIleGlnAsnMetIleValGluGluCysGlyCysSer

일반 설명

Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin A has two 116 amino acids residues βA subunits (βA-βA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.
As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.

물리적 형태

Lyophilized from a Tris HCl 0.05 M buffer at pH 7.4

재구성

Lyophilized protein should be reconstituted in water to a concentration of 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.

분석 메모

The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation.
ED₅₀: ≤ 5 ng/mL

기타 정보

Extinction Coefficient: E^0.1% = 1.27 (A ₂₈₀ nm)
p.I: 7.27

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable