추천 제품
생물학적 소스
bovine
Quality Level
제품 라인
BioReagent
분석
≥98%
양식
lyophilized powder
분자량
~66 kDa
정제법
heat shock fractionation
포장
poly bottle of
기원
USA origin
기술
cell culture | mammalian: suitable
불순물
protease, none detected
≤1.0 EU/mg endotoxin
손실
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt 수납 번호
외래 활성
BT virus, none detected
Protease by FITC, none detected
VSV virus, none detected
저장 온도
2-8°C
유전자 정보
bovine ... ALB(280717)
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일반 설명
Bovine serum albumin (BSA), a 66 kDa protein, belongs to the family of serum albumins. It is an α-helical, non-glycosylated and globular protein, that has 17-disulfide bonds.
애플리케이션
Bovine Serum Albumin has been used:
- as a component of cell culture media in cochlear explant culture
- organotypic cultures and embryo culture
- as a component of phosphate-buffered saline (PBS) in immunostaining of human induced pluripotent stem cell (hiPSC)
생화학적/생리학적 작용
Bovine Serum Albumin (BSA) is the most vital constituent of the cell culture media, which aids embryonic stem cells (hESC) differentiation. It acts as a blocking agent in enzyme linked immunosorbent assay (ELISA) to inhibit non-specific binding of antigens and antibodies to the microtiter plates.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
특징 및 장점
- Tested for use in cell culture
- Protease-free
- Low endotoxin
제조 메모
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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Lot/Batch Number
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