A6778

Angiotensin Converting Enzyme from rabbit lung

≥2.0 units/mg protein (modified Warburg-Christian)

• 동의어(들): ACE, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase
• CAS Number: 9015-82-1
• EC 번호: 3.4.15.1 (BRENDA, IUBMB)
• MDL number: MFCD00130325
• UNSPSC 코드: 12352204
• eCl@ss: 42010109
• NACRES: NA.32

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥2.0 units/mg protein (modified Warburg-Christian)
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site. ACE regulates blood pressure through renin-angiotensin system. ACE elevates blood pressure by converting angiotensin I to a key vasoconstrictor angiotensin II and inhibiting a potent vasodilator bradykinin. Inhibition of ACE is a targeted therapeutic strategy for high blood pressure. Several ACE synthetic inhibitory peptides available for clinical use include captopril, enalapril and lisinopril. Currently, developing inhibitory peptides from natural food sources, or phenolic compounds from plant sources to inhibit ACE is underway. ACE plays a critical role in fertilization by releasing the proteins anchored to glycosylphosphatidylinositol (GPI) in sperm membrane.

애플리케이션

Angiotensin converting enzyme from rabbit lung has been used:

• for measuring inhibitory effect of egg white protein hydrolysates on ACE activity by high performance liquid chromatography (HPLC)
• to measure the ACE inhibition by litchi pericarp and cooked chicken breast using hippuryl-L-histidyl-L-leucine (HHL) as substrate by reverse phase-HPLC (RP-HPLC)3 and HPLC respectively
• in releasing GPI anchored protein in vitro in few cell lines like HeLa, HEK293 and in vivo in mice sperm.

생화학적/생리학적 작용

Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.

품질

May contain traces of sodium chloride.

단위 정의

One unit will produce 1.0 μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)