A7058
Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal
clone HIS-1, purified from hybridoma cell culture
동의어(들):
Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
생물학적 소스
mouse
Quality Level
결합
peroxidase conjugate
항체 형태
purified from hybridoma cell culture
항체 생산 유형
primary antibodies
클론
HIS-1, monoclonal
양식
lyophilized powder
포장
vial of 0.5 mL
농도
5-11 mg/mL
기술
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
동형
IgG2a
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
특이성
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
면역원
recombinant polyHistidine tagged fusion protein.
애플리케이션
Antibody suitable for immunoblotting. Working dilution 1:20
Also suitable for dot blot assays and ELISA
Also suitable for dot blot assays and ELISA
물리적 형태
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
제조 메모
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
재구성
The antibody conjugate should be reconstituted with 0.5 ml deionized water.
법적 정보
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable