A7510

Adenosine 5′-Triphosphatase from porcine cerebral cortex

lyophilized powder, ≥0.3 units/mg protein, pH 7.8

• 동의어(들): ATP phosphohydrolase, ATPase
• CAS Number: 9000-83-3
• EC 번호: 3.6.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00135671
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Porcine cerebral cortex
• Quality Level: 200
• 설명: Sodium and Potassium-Activated
• 양식: lyophilized powder
• 특이 활성도: ≥0.3 units/mg protein, pH 7.8
• 구성: Protein, 5.0-25.0% biuret
• 기술: inhibition assay: suitable
• UniProt 수납 번호: P05024; P05027
• 저장 온도: −20°C
• 유전자 정보: pig ... ATP1A1(397481), ATP1B1(396898)

설명

애플리케이션

ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity .

생화학적/생리학적 작용

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na⁺,K⁺-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA).

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells .

단위 정의

One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na⁺, K^+, and Mg²⁺.

물리적 형태

Lyophilized powder containing 90% sucrose, 0.4% EDTA Na₄ and 0.06% NaCl

제조 메모

Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)