A7653

L-Alanine Dehydrogenase from Bacillus subtilis

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

• 동의어(들): L-Alanine: NAD⁺ oxidoreductase (deaminating)
• CAS Number: 9029-06-5
• EC 번호: 1.4.1.1 (BRENDA, IUBMB)
• EC Number: 232-847-9
• MDL number: MFCD00131441
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Bacillus subtilis
• Quality Level: 200
• 양식: buffered aqueous glycerol solution
• 특이 활성도: ~30 units/mg protein (Lowry)
• 외래 활성: LDH ~1% (using pyruvate as substrate)
• 저장 온도: −20°C

설명

애플리케이션

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .

생화학적/생리학적 작용

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

단위 정의

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH₃ per min at pH 10.0 at 25 °C.

물리적 형태

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)