C3934

Carbonic Anhydrase from bovine erythrocytes

lyophilized powder, ≥2,000 W-A units/mg protein

• 동의어(들): Carbonate Dehydratase, Carbonate Hydrolyase
• CAS Number: 9001-03-0
• EC 번호: 4.2.1.1 (BRENDA, IUBMB)
• EC Number: 232-576-6
• MDL number: MFCD00081467
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine erythrocytes
• 양식: lyophilized powder
• 특이 활성도: ≥2,000 W-A units/mg protein
• 분자량: 30 kDa
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: 2-8°C

설명

애플리케이션

Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn²⁺, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO₂ measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.

생화학적/생리학적 작용

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO₂ homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson′s disease.

단위 정의

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)