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Merck
모든 사진(2)

주요 문서

C6891

Sigma-Aldrich

5-Chloro-2′-deoxyuridine

thymidine analog

동의어(들):

CldU

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About This Item

실험식(Hill 표기법):
C9H11ClN2O5
CAS Number:
Molecular Weight:
262.65
MDL number:
UNSPSC 코드:
41106305
PubChem Substance ID:
NACRES:
NA.51

Quality Level

분석

≥98% (HPLC)

양식

powder

solubility

1 M NH4OH: 20 mg/mL, clear, colorless to faintly yellow

저장 온도

−20°C

SMILES string

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Cl)C(=O)NC2=O

InChI

1S/C9H11ClN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

NJCXGFKPQSFZIB-RRKCRQDMSA-N

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애플리케이션

5-Chloro-2′-deoxyuridine has been used to:
  • study cell cycle dynamics by immunohistochemical analysis and double S-phase labeling using animal models
  • analyze cell cycle length using an animal model
  • label HEK293T cells for molecular combing assay

생화학적/생리학적 작용

DNA labeled with 5-chloro-2′-deoxyuridine (CldU) serves as an effective tool to analyze and quantify DNA replication, repair, and recombination. CldU is a potent mutagen, clastogen, and toxicant. It is used as a thymidine analog and is found to alter the dNTP pools and might lead to cell-cycle arrest. CldU produces sister-chromatid exchange but has less response to ionizing radiation compared to other thymine analogs. 5-Chloro-2′-deoxyuridine (CldU) is used to study the miscoding potential of hypochlorous acid damage to DNA and DNA precursors. When used with antibody based immunofluorescent imaging, 5-Chloro-2′-deoxyuridine incorporation may be used in protocols to identify sites of DNA replication. CldU may be used as a labeling substrate in conjunction with other halogenated uridine labeling substrates such as iododeoxyuridine (IdU).

픽토그램

Health hazardExclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


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