C8554

Chorionic gonadotropin human

lyophilized powder, suitable for mouse embryo cell culture

• 동의어(들): Choriogonin, HCG
• CAS Number: 9002-61-3
• EC Number: 232-660-2
• MDL number: MFCD00130797
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human urine
• Quality Level: 200
• 무균: aseptically processed
• 양식: lyophilized powder
• 효능: ≥5,000 I.U. per mg
• 분자량: 37.9 kDa; apparent mol wt 37.9 kDa by SDS-PAGE
• drug control: Home Office Schedule 4.2
• 기술: cell culture | embryo: suitable
• 불순물: HBV,HCV and HIV, tested negative
• solubility: H₂O: 1 mL/vial; saline: 1 mL/vial
• UniProt 수납 번호: P01215
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... CGA(1081)

설명

일반 설명

Glycoprotein hormone consisting of a 92-amino acid α-chain which is identical to that of LH, FSH, and TSH; and a distinct 149-amino acid β-chain. Produced by chorionic tissue and responsible for maintaining the corpus luteum during early pregnancy. Also produced by trophoblast cell neoplasms, which are marked by elevated free hCG β-chains and hyperglycosylated hCG.

Note: Actual molecular weight will vary from the theoretical depending upon the degree of glycosylation. Theoretical MW is 37.9 kDa based on native form which contains 2 subunits: α = 14.9 kDa (~10.2 kDa for the polypeptide and ~4.7 kDa for the carbohydrate), and β = 23.0 kDa (~16.0 kDa for the polypeptide and ~7.0 kDa for the carbohydrate). See J. Biol. Chem. 248, 6796-6827 (1973).

애플리케이션

Recommended for superovulation induction in mice.

생화학적/생리학적 작용

Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.

Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.

안전성 정보

• 픽토그램: GHS08
• 신호어: Warning
• 유해 및 위험 성명서: H361
• 예방조치 성명서: P281
• Hazard Classifications: Repr. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable