C8726

Caspase 9 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous solution, >2,000 units/mg protein

• 동의어(들): ICE-Lap6, Mch6
• EC 번호: 3.4.22.62 (BRENDA, IUBMB)
• MDL number: MFCD02687006
• UNSPSC 코드: 12352204

속성

• 재조합: expressed in E. coli (C-terminal histidine-tagged)
• Quality Level: 200
• 분석: ≥90% (SDS-PAGE)
• 양식: buffered aqueous solution
• 특이 활성도: >2,000 units/mg protein
• 분자량: N-terminal prodomain plus the large subunit. 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein); small subunit 13 kDa (subunit containing the histidine tag)
• UniProt 수납 번호: P55211
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CASP9(842)

설명

생화학적/생리학적 작용

Activation of caspase-9 (CASP9) through apoptotic stimuli initiates the caspase cascade. Caspases have been implicated in many disorders including cancer, inflammatory disease, neurodegenerative diseases, stroke and myocardial infarction.

When cells receive apoptotic stimuli, such as activation of the TNFα/Fas cell surface receptor, caspase 8 activation, Bid processing and its translocation to the mitochondria ensue. As a result mitochondria release cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP. The resultant complex recruits caspase 9 leading to its activation. It then cleaves downstream caspases such as caspase 3, 6, and 7, initiating the caspase cascade. Caspase 9 exhibits basal activity. It can be further activated via interaction of its N-terminal prodomain with the activator protein Apaf-1 in the presence of cytochrome c and dATP. Caspases have been implicated in many disorders including cancer, inflammatory disease, neurodegenerative diseases, stroke and myocardial infarction.

단위 정의

One unit will cleave 1.0 nmol of Ac-Leu-Glu-His-Asp-AFC per hr at 25 °C at pH 6.5.

물리적 형태

Solution in 10% sucrose containing 50 mM HEPES, pH 7.5, 5 mM DTT, 0.1% CHAPS, 1 mM EDTA, 50 mM NaCl.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)