C8868

Cholesterol Oxidase from microorganisms

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

• 동의어(들): Cholesterol: oxygen oxidoreductase
• CAS Number: 9028-76-6
• EC 번호: 1.1.3.6 (BRENDA, IUBMB)
• EC Number: 232-842-1
• MDL number: MFCD00130783
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: ≥50 units/mg protein
• 분자량: 64 kDa
• 구성: Protein, ≥15% biuret
• 저장 온도: −20°C

설명

애플리케이션

Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.

Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

생화학적/생리학적 작용

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H₂O₂.

물리적 특성

Type II cholesterol oxidase containing FAD cofactor covalently linked to the enzyme.

단위 정의

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

물리적 형태

Supplied as a lyophilzed powder containing sucrose

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)