C9233

Monoclonal Anti-Chk2 antibody produced in mouse

clone DCS-270, purified from hybridoma cell culture

• MDL number: MFCD03457550
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: DCS-270, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen 61 kDa
• 종 반응성: human
• 농도: ~1 mg/mL
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1-2 μg/mL using using whole extract of cultured 293T (human embryonal kidney) cells
• 동형: IgG2a
• UniProt 수납 번호: O96017
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CHEK2(11200)

설명

일반 설명

The protein encoded by this gene is a serine/threonine-protein kinase that acts as a cell cycle checkpoint regulator and a putative tumor suppressor. It contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA), which is activated by rapid phosphorylation in response to replication blocks and DNA damage.

특이성

The epitope recognized by the antibody resides within the N-terminal regulatory domain of the Chk2 molecule.

면역원

recombinant human Chk2.

애플리케이션

Monoclonal Anti-Chk2 antibody produced in mouse is suitable for immunocytochemistry, immunohistochemistry (formalin-fixed, paraffin-embedded sections), microarray, immunoprecipitation and for immunoblotting at a concentration of 1-2 μg/mL using whole extract of cultured 293T cells. It was used for Chk2 immunoprecipitation experiments to study the engagement of the ATR-dependent DNA damage response at the human papillomavirus 18 replication centers during the initial amplification

생화학적/생리학적 작용

The protein propagates signals from damaged or unreplicated DNA. It is expressed throughout the cell cycle. Upon activation it phosphorylates and inhibits CDC25C, which leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, blocking cell cycle progression. It also regulates apoptosis through the phosphorylation of p53, a tumor suppressor protein. The protein regulates DNA repair through phosphorylation of BRCA2. Mutations in this gene have been linked to Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable