추천 제품
Quality Level
사용
sufficient for 100 tests
유통기한
Kit is stable for 24 months when unopened.
검출 방법
colorimetric
배송 상태
wet ice
저장 온도
−20°C
일반 설명
The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.
적합성
Suitable for the measureent of glutathione peroxidase in tissue extracts
원리
The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.
기타 정보
This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.
키트 구성품 전용
제품 번호
설명
- tert-Butyl hydroperoxide 1 mL
신호어
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
5.2 - Organic peroxides and self-reacting hazardous materials
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