CS1070

Transglutaminase Assay Kit

sufficient for assays in two 96-well plates

• 동의어(들): TGases Assay Kit
• UNSPSC 코드: 12161503
• NACRES: NA.28

속성

• Quality Level: 200
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... TGM1(7051), TGM2(7052), TGM3(7053), TGM4(7047), TGM5(9333), TGM6(343641), TGM7(116179)

설명

일반 설명

The Transglutaminase Assay Kit is designed for the detection of transglutaminase activity in biological samples. The kit can also be used for screening of transglutaminase inhibitors/activators.

애플리케이션

The assay is based on transglutaminase catalysis of a covalent bond formation between the free amine group of Poly L-Lysine, which is covalently attached to the plate surface, and the γ-carboxamide group of biotin-TVQQEL-OH substrate present in the assay buffer. This reaction immobilizes biotin to the plate surface. The amount of immobilized biotin is proportional to the amount of active transglutaminase in the sample. The amount of immobilized biotin is determined using streptavidin-peroxidase and TMB substrate.

생화학적/생리학적 작용

Transglutaminases (TGases) are a widely distributed and unique group of calcium dependent enzymes that catalyze the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The cross-linked products, often of high molecular mass, are highly resistant to mechanical challenge and proteolytic degradation, and their accumulation is found in a number of tissues and processes, where such properties are important including skin, hair, blood clotting, and wound healing. Deregulation of the enzyme activity contributes to a number of human diseases.

기타 정보

The detection limit is 0.003 mU/assay or 0.03mU/ml of Transglutaminase.

키트 구성품 역시 별도로 이용 가능함

• T5398 Transglutaminase from guinea pig liver, lyophilized powder, ≥1.5 units/mg protein 2 ea
• T0440 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate
• S5512 Streptavidin−Peroxidase from Streptomyces avidinii, lyophilized powder 100 μg

안전성 정보

• 픽토그램: GHS08,GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H290,H315,H318,H334,H412
• 예방조치 성명서: P234 - P261 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2
• Storage Class Code: 8A - Combustible corrosive hazardous materials