CY0100

Cytochrome c Reductase (NADPH) Assay Kit

1 kit sufficient for 100 tests, determining cytochrome c reductase activity

• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• Quality Level: 200
• 사용: kit sufficient for 100 tests
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... POR(5447)

설명

생화학적/생리학적 작용

Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.

특징 및 장점

• Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation
• Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics
• Analyze enrichment and function of ER

분석 메모

The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.

키트 구성품 전용

• Assay Buffer 100 mL
• Enzyme Dilution Buffer 25 mL
• Cytochrome c 50 mg
• Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
• Cytochrome c Oxidase Inhibitor Solution 1 mL

키트 구성품 역시 별도로 이용 가능함

• N6505 β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H301,H412
• 예방조치 성명서: P264 - P270 - P273 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Chronic 3
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects