D218

Monoclonal Anti-Dihydropyridine Receptor (α₁ Subunit) antibody produced in mouse

clone 1A, buffered aqueous solution

• MDL number: MFCD01633297
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 결합: unconjugated
• Quality Level: 200
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 1A, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~200 kDa
• 종 반응성: human (weakly), guinea pig, mouse, rat, rabbit
• 기술: immunohistochemistry (frozen sections): 1:200; immunoprecipitation (IP): suitable; western blot (chemiluminescent): 1:500
• 동형: IgG1
• UniProt 수납 번호: Q13698
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CACNA1S(779) ; mouse ... Cacna1s(12292) ; rat ... Cacna1s(682930)

설명

일반 설명

DHP receptor is composed of at least four subunits. The α1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated Na+ channel. The α2 subunit is a large glycoprotein and the other 2 subunits are termed β and γ.

특이성

Reacts specifically with the α₁ subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

면역원

rabbit muscle transverse tubule dihydropyridine (DHP) receptors.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse is suitable for immunoprecipitation, immunohistochemistry at a working dilution of 1:500 and immunoblotting at 1:2000. It was used for the detection and localization of DHPR-1α by western blotting in skeletal muscle tissue sections of mice in a study. The antibody may be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.

생화학적/생리학적 작용

The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type Ca²⁺ channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.

물리적 형태

Solution in phosphate buffered saline containing 0.05% sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)