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Merck
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DUO92105

Sigma-Aldrich

Duolink® In Situ Red Starter Kit Goat/Rabbit

동의어(들):

in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit

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모든 사진(4)

About This Item

UNSPSC 코드:
41116158
NACRES:
NA.32

제품 라인

Duolink®

기술

proximity ligation assay: suitable

형광

λex 594 nm; λem 624 nm (red) (Texas Red®; Zeiss Filter set 31)

적합성

suitable for fluorescence

관련 카테고리

애플리케이션

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Goat PLUS and Anti-Rabbit MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Red Starter Kit Goat/Rabbit requires one primary antibody from goat and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

특징 및 장점

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

법적 정보

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • DUO92005Duolink® In Situ PLA® Probe Anti-Rabbit MINUSSDS
  • DUO92003Duolink® In Situ PLA® Probe Anti-Goat PLUSSDS
  • DUO92008Duolink® In Situ Detection Reagents RedSDS
  • DUO82040Duolink® In Situ Mounting Medium with DAPISDS
  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS

픽토그램

Health hazardCorrosion
GHS08,GHS05

신호어

Danger

유해 및 위험 성명서

H290,H334,H412

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Resp. Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number

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