E0287

Carboxylesterase 1 isoform b human

recombinant, expressed in baculovirus infected BTI insect cells

• 동의어(들): Carboxylesterase 1 human, carboxylesterase, esterase
• EC 번호: 3.1.1.1 (BRENDA, IUBMB)
• EC Number: 232-773-7
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in baculovirus infected BTI insect cells
• Quality Level: 200
• 양식: liquid
• 특이 활성도: ≥500 units/mg protein
• 농도: 5 mg/mL
• 중량: (0.5 ml)
• 배송 상태: dry ice
• 저장 온도: −70°C

설명

일반 설명

Carboxylesterase (CE) belongs to serine hydrolase family. It is widely present in the human body. Five isoforms of carboxylesterase is present in humans. It is encoded by CES1 gene mapped to 16q22.2 in human chromosome.

Carboxylesterase 1 is a member of a large multigene carboxylesterase family. These enzymes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolyze long-chain fatty acid esters and thioesters. This enzyme is known to hydrolyze aromatic and aliphatic esters and is necessary for cellular cholesterol esterification. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds.

애플리케이션

Carboxylesterase 1 isoform b human has been used in the reaction to convert Amplex red to resorufin in horse radish peroxidase and oxygen independent manner. It has also been used in the development of an emission ratiometric two-photon probe (SE1) for quantitation in situ.

Delivers high catalytic activity, ideal for robust high-throughput screening assays including drug-drug interaction studies, and pharmacokinetic studies for evaluating pro-drugs and non-CYP pathways of elimination.

생화학적/생리학적 작용

Carboxylesterase (CE) is implicated in drug metabolism in liver and hepatic steatosis. Deficiency of CE in humans causes obesity, hyperlipidemia, insulin insensitivity, and cancer.

단위 정의

One unti will hydrolyze one nanomole of 4-nitrophenyl acetate per minute at pH 7.4 at 37°C

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable