E6654

EZview^™ Red Anti-c-Myc Affinity Gel

• 동의어(들): Anti-c-Myc affinity resin, Anti-c-Myc agarose beads
• UNSPSC 코드: 12352203
• NACRES: NA.56

속성

• 유통기한: 1 yr at 2‑8 °C
• Quality Level: 200
• 분석물 화학적 분류: proteins (c-Myc)
• 기술: affinity chromatography: suitable; immunoprecipitation (IP): suitable
• 용량: ~0.05 mg/mL binding capacity
• 저장 온도: 2-8°C

설명

일반 설명

EZ view Red Anti--c-Myc Affinity Gel is a resin that consists of the Anti-c-Myc rabbit antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind c-Myc fusion proteins to samples, such as cell lysates and tissue, for purification of c-Myc-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
Agarose beads bind at N-terminal, Met-N-terminal and C-terminal c-Myc fusion proteins from E. coli and mammalian cells.

애플리케이션

EZview^™ Red Anti-c-Myc Affinity Gel is suitable for:

• SUMOylation assay
• Affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

특징 및 장점

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

기타 정보

The affinity gel contains affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to beaded agarose. The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein.

물리적 형태

1:1 (v/v) suspension in PBS containing 50% glycerol and 0.0015% Kathon CG/IPCII as an antimicrobial preservative

법적 정보

EZview is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable