F2055

Anti-hFBXW7 antibody , Mouse monoclonal

clone FB407, purified from hybridoma cell culture

• 동의어(들): Anti-ARCHIPELAGO (Drosophila), Anti-Cdc4 (S. serevisia), Anti-F-box and WD40 domain protein 7, Anti-FBW7, Anti-SEL-10 (C. elegance)
• MDL number: MFCD09265423
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: FB407, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~60 kDa
• 종 반응성: rat, human, mouse
• 농도: ~2 mg/mL
• 기술: immunocytochemistry: 10-20 μg/mL using human A431 or HeLa cells; indirect ELISA: suitable; western blot: 5-10 μg/mL using human A431 cell extracts
• 동형: IgG2a
• UniProt 수납 번호: Q969H0
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FBXW7(55294) ; mouse ... Fbxw7(50754)

설명

일반 설명

FBXW7 is a member of F-box protein family, which acts as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). The proteins have a bipartite structure. The shared F-box motif links F-box protein to Skp1 and the core complex, whereas divergent protein-protein interaction motifs selectively bind their cognate substrates. There are three FBXW7 isoforms α, β and γ that share 10 out of 11 exons but they differ in their subcellular localization as well as in substrate recognition activity.

Monoclonal Anti-hFBXW7 (mouse IgG2a isotype) is derived from the hybridoma FB407 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino-acids of human hFBXW7 isoforms α β and γ. FBXW7 is located on human chromosome 4q31.

특이성

The antibody recognizes human, mouse, and rat hFBXW7.

면역원

synthetic peptide corresponding to amino-acids 238-250 of human hFBXW7 isoforms α, β, and γ.

애플리케이션

Anti-hFBXW7 antibody, Mouse monoclonal has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

생화학적/생리학적 작용

F-box proteins is associated with various signalling pathways, such as nutrient sensing in yeast,conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch, leading to their ubiquitination and degradation. Furthermore, it was found mutated in breast, endometrial, ovarian, and colon cancer, and finally results in genomic instability. Similar to the inactivation mode of other known tumor suppressors, the SV40 large T antigen binds FBXW7 without detectible effects on its stability, and thus acts as an inhibitor of FBXW7 activity.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)