F4051

Proteasome Fraction II from rabbit

5 mg/mL, Reticulocyte cell extract, solution

• MDL number: MFCD03458197
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• Quality Level: 200
• 양식: solution
• 농도: 5 mg/mL
• 저장 온도: −70°C

설명

일반 설명

Manufactured for Sigma by Boston Biochem., Inc.

애플리케이션

Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.

생화학적/생리학적 작용

Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.

Protein fraction of a cell extract that binds to anion-exchange resin.

물리적 형태

Solution in 25 mM HEPES, pH 7.6.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable